pSV277 dna sequence 
Clone into 5'-BamHI site

cgatcccgcgaaattaatacgactcactataggggaattgtgagcggata
acaattcccctctagaaataattttgtttaactttaagaaggagatataC
ATATGcggggttctcatcatcatcatcatcatggtatggctagcatgact
ggtggacagcaaatgggtcgggatctgtacgacgatgacgatctggttcc
gcgtGGATCCCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCG
AGatcaaacgggctagccagccagaactcgccccggaagaccccgaggat
gtcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccg
aaaggaagctgagttggctgctgccaccgctgagcaataactagcataac
cccttggggcctctaaacgggtcttgaggggttttttgctgaaaggagga
actatatccggattggcgaatgggacgcgccctgtagcggcgcattaagc
gcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgc
cctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcg
ccggctttccccgtcaagctctaaatcgggggctccctttagggttccga
tttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatgg
ttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgt
tggagtccacgttctttaatagtggactcttgttccaaactggaacaaca
ctcaaccctatctcggtctattcttttgatttataagggattttgccgat
ttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcga
attttaacaaaatattaacgtttacaatttcaggtggcacttttcgggga
aatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatat
gtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatga
aactgcaatttattcatatcaggattatcaataccatatttttgaaaaag
ccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatgg
caagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaa
cctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcacc
atgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctt
tccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactc
gcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaa
tacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaacc
ggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcagga
tattcttctaatacctggaatgctgttttcccggggatcgcagtggtgag
taaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagag
gcataaattccgtcagccagtttagtctgaccatctcatctgtaacatca
ttggcaacgctacctttgccatgtttcagaaacaactctggcgcatcggg
cttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgc
gagcccatttatacccatataaatcagcatccatgttggaatttaatcgc
ggcctagagcaagacgtttcccgttgaatatggctcataacaccccttgt
attactgtttatgtaagcagacagttttattgttcatgaccaaaatccct
taacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa
aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaa
caaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagcta
ccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaa
tactgtccttctagtgtagccgtagttaggccaccacttcaagaactctg
tagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgct
gccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt
accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagc
ccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgag
ctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatcc
ggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggg
gaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgactt
gagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa
cgccagcaacgcggcctttttacggttcctggccttttgctggccttttg
ctcacatgttctttcctgcgttatcccctgattctgtggataaccgtatt
accgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcg
cagcgagtcagtgagcgaggaagcggaagagcgcctgatgcggtattttc
tccttacgcatctgtgcggtatttcacaccgcatatatggtgcactctca
gtacaatctgctctgatgccgcatagttaagccagtatacactccgctat
cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgct
gacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagc
tgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcac
cgaaacgcgcgaggcagctgcggtaaagctcatcagcgtggtcgtgaagc
gattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctc
cagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg
ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttc
atgggggtaatgataccgatgaaacgagagaggatgctcacgatacgggt
tactgatgatgaacatgcccggttactggaacgttgtgagggtaaacaac
tggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgc
cagcgcttcgttaatacagatgtaggtgttccacagggtagccagcagca
tcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg
tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgct
caggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtat
cggtgattcattctgctaaccagtaaggcaaccccgccagcctagccggg
tcctcaacgacaggagcacgatcatgcgcacccgtggggccgccatgccg
gcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgac
gaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc
cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccag
agcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcat
aagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactg
ggttgaaggctctcaagggcatcggtcgagatcccggtgcctaatgagtg
agctaacttacattaattgcgttgcgctcactgcccgctttccagtcggg
aaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag
gcggtttgcgtattgggcgcAagggtggtttttcttttcaccagtgagac
gggcaacagctgattgcccttcaccgcctggccctgagagagttgcagca
agcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtg
gttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccac
taccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgca
ttgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg
atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcact
ccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagat
atttatgccagccagccagacgcagacgcgccgagacagaacttaatggg
cccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccac
gcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtg
tctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct
tccacagcaatggcatcctggtcatccagcggatagttaatgatcagccc
actgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcga
cgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcg
gcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccag
actggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgtt
gtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact
ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcggga
aacggtctgataagagacaccggcatactctgcgacatcgtataacgtta
ctggtttcacattcaccaccctgaattgactctcttccgggcgctatcat
gccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgac
gctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttg
aggccgttgagcaccgccgccgcaaggaatggtGCATGCgcaacgccaat
cagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgt
aattcagctccgccatcgccgcttccactttttcccgcgttttcgcagaa
acgtggctggcctggttcaccacgcgggaaacggtctgataagagacacc
ggcatactctgcgacatcgtataacgttactggtttcacAGATCT