pNR114 dna sequence
Kan resistant; clone into BamHI site

tcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagaa
ataattttgtttaactttaagaaggagatataCATATGggttcttctcaccaccaccaccacca
tcaccatcaccatggcagcagcctggttccgcgtGGATCCccGAATTCGAGCTCcGTCGACAAG
CTTGCGGCCGCaCTCGAGatcaaacgggctagccagccagaactcgccccggaagaccccgagg
atgtcgagGGTACCcatcaccatcaccaccaccaccactgagatccggctgctaacaaagcccg
aaaggaagctgagttggctgctgccaccgctgagcaataactagcataaccccttggggcctct
aaacgggtcttgaggggttttttgctgaaaggaggaactatatccggattggcgaatgggacgc
gccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacactt
gccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggct
ttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacct
cgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtt
tttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaa
cactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattg
gttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttaca
atttcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac
attcaaatatgtatccgctcatgaattaattcttagaaaaactcatcgagcatcaaatgaaact
gcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaagg
agaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgact
cgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaat
caccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttg
ttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcatt
cgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaa
tcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggata
ttcttctaatacctggaatgctgttttcccggggatcgcagtggtgagtaaccatgcatcatca
ggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctga
ccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgc
atcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccat
ttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttccc
gttgaatatggctcataacaccccttgtattactgtttatgtaagcagacagttttattgttca
tgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa
aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccg
ctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggct
tcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaa
gaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagt
ggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggt
cgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgag
atacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtat
ccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggt
atctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtc
aggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgc
tggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccg
cctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcga
ggaagcggaagagcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgc
atatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccg
ctatcgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct
gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcat
gtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagctcatcagcg
tggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgttgagtttctcca
gaagcgttaatgtctggcttctgataaagcgggccatgttaagggcggttttttcctgtttggt
cactgatgcctccgtgtaagggggatttctgttcatgggggtaatgataccgatgaaacgagag
aggatgctcacgatacgggttactgatgatgaacatgcccggttactggaacgttgtgagggta
aacaactggcggtatggatgcggcgggaccagagaaaaatcactcagggtcaatgccagcgctt
cgttaatacagatgtaggtgttccacagggtagccagcagcatcctgcgatgcagatccggaac
ataatggtgcagggcgctgacttccgcgtttccagactttacgaaacacggaaaccgaagacca
ttcatgttgttgctcaggtcgcagacgttttgcagcagcagtcgcttcacgttcgctcgcgtat
cggtgattcattctgctaaccagtaaggcaaccccgccagcctagccgggtcctcaacgacagg
agcacgatcatgcgcacccgtggggccgccatgccggcgataatggcctgcttctcgccgaaac
gtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaag
cgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgct
gccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca
tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgagatcc
cggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctttccagtcg
ggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta
ttgggcgcaagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccg
cctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctg
tttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactacc
gagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatct
gatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttg
aaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtg
agatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaaca
gcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatg
ggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacatta
gtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccac
tgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctac
catcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc
gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgcca
gttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccg
cgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccg
gcatactctgcgacatcgtataacgttactggtttcacattcaccaccctgaattgactctctt
ccgggcgctatcatgccataccgcgaaaggttttgcgccattcgatggtgtccgggatctcgac
gctctcccttatgcgactcctgcattaggaagcagcccagtagtaggttgaggccgttgagcac
cgccgccgcaaggaatggtgcatgcgcaacgccaatcagcaacgactgtttgcccgccagttgt
tgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccactttttcccgcgttt
tcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctgataagagacaccggcata
ctctgcgacatcgtataacgttactggtttcacagatct