pS219V

Allows expression of N-terminally His-tagged TEV protease in E. coli.
TEV protease recognition sequence: ENLYFQ↓G

• Ref: Protein Eng. 14:993-1000 (2001)
• tac promoter
• low copy
• Amp resistance
• Encodes Ser219 → Val point mutant of the TEV protease catalytic domain
• S219V mutant has been shown to be more stable and more efficient as a catalyst than the wild-type protease
• pS219V produces an MBP-His-TEV fusion protein with an internal cleavage site
• The product self-cleaves intracellularly to yield His-tagged protease, MW = 28.5 kDa