LOCUS       pKLPBac                 5849 bp    DNA     circular SYN 12-SEP-2012
DEFINITION  .
ACCESSION   pKLPBac
KEYWORDS    .
SOURCE      Unknown.
  ORGANISM  Unknown
            Unclassified.
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Self
  JOURNAL   Unpublished.
COMMENT     SECID/File created by Clone Manager, Scientific & Educational Software
COMMENT     SECNOTES|GenBank       5849 bp ds-DNA     circular     12-SEP-2012
            ACCESSION   
            VERSION     
FEATURES             Location/Qualifiers
     misc_feature    complement(1..816)
                     /label=KanR
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=#ffff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="KanR"
     rep_origin      862..1544
                     /label=ColE1 origin
                     /ApEinfo_fwdcolor=gray50
                     /ApEinfo_revcolor=gray50
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="ColE1 origin"
     misc_feature    1831..2055
                     /label=Tn7R
                     /ApEinfo_fwdcolor=#0064fc
                     /ApEinfo_revcolor=#0064fc
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tn7R"
     misc_feature    complement(2120..2872)
                     /label=GentR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Gene"
                     /SECStyleId=1
                     /SECName="GentR"
     misc_recomb     3268..3301
                     /label=LoxP
                     /ApEinfo_fwdcolor=steel blue
                     /ApEinfo_revcolor=orange
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="LoxP"
     misc_feature    3333..3461
                     /label=Polh promoter
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Polh promoter"
     misc_feature    3474..3476
                     /label=Start
                     /ApEinfo_fwdcolor=#d6e9ff
                     /ApEinfo_revcolor=#d6e9ff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Start"
     misc_feature    3480..4205
                     /label=TEV protease
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=cyan
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TEV protease"
     misc_feature    4245..4265
                     /label=TEV site1
                     /ApEinfo_fwdcolor=#feff0c
                     /ApEinfo_revcolor=#feff0c
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="TEV site1"
     misc_feature    4266..5009
                     /label=eCFP
                     /ApEinfo_fwdcolor=#00ff13
                     /ApEinfo_revcolor=#00ff13
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="eCFP"
     polyA_signal    5102..5293
                     /label=SV40 late polyA
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="SV40 polyA"
     misc_feature    5328..5493
                     /label=Tn7L
                     /ApEinfo_fwdcolor=#015ab8
                     /ApEinfo_revcolor=#015ab8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /SECDrawAs="Region"
                     /SECStyleId=1
                     /SECName="Tn7L"
ORIGIN      
        1 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
       61 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
      121 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
      181 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
      241 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
      301 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
      361 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
      421 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
      481 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
      541 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
      601 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
      661 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac
      721 attatctcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
      781 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
      841 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
      901 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
      961 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1021 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1081 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1141 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1201 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1261 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1321 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1381 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1441 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1501 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1561 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1621 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1681 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     1741 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     1801 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     1861 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     1921 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     1981 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2041 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2101 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2161 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     2221 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     2281 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     2341 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc
     2401 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     2461 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     2521 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     2581 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     2641 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     2701 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     2761 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     2821 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     2881 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     2941 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3001 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc
     3061 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3121 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3181 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca
     3241 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta
     3301 tctggtttcg acctactccg gaatattaat agatcatgga gataattaaa atgataacca
     3361 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
     3421 aaatattccg gattattcat accgtcccac catcgggcgc ggatcccatg ggcatgggcg
     3481 gagaaagctt gtttaaggga ccacgtgatt acaacccgat atcgagcacc atttgtcatt
     3541 tgacgaatga atctgatggg cacacaacat cgttgtatgg tattggattt ggtcccttca
     3601 tcattacaaa caagcacttg tttagaagaa ataatggaac actgttggtc caatcactac
     3661 atggtgtatt caaggtcaag aacaccacga ctttgcaaca acacctcatt gatgggaggg
     3721 acatgataat tattcgcatg cctaaggatt tcccaccatt tcctcaaaag ctgaaattta
     3781 gagagccaca aagggaagag cgcatatgtc ttgtgacaac caacttccaa actaagagca
     3841 tgtctagcat ggtgtcagac actagttgca cattcccttc atctgatggc atattctgga
     3901 agcattggat tcaaaccaag gatgggcagt gtggcagtcc attagtatca actagagatg
     3961 ggttcattgt tggtatacac tcagcatcga atttcaccaa cacaaacaat tatttcacaa
     4021 gcgtgccgaa aaacttcatg gaattgttga caaatcagga ggcgcagcag tgggttagtg
     4081 gttggcgatt aaatgctgac tcagtattgt gggggggcca taaagttttc atgagcaaac
     4141 ctgaagagcc ttttcagcca gttaaggaag cgactcaact catgaatgaa ttggtgtact
     4201 cgcaaggtaa ccatagggat aacagggtaa tgcggtccgg tggtgaaaac ctgtattttc
     4261 agggcgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4321 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4381 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4441 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga
     4501 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4561 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4621 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4681 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga
     4741 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4801 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4861 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4921 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4981 ccggactcag atctcgacga gctcactagt aatctagagc ctgcagtctc gacaagcttg
     5041 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     5101 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     5161 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5281 cttatcatgt ctggatctga tcactgcttg agcgcctagg agatccgaac cagataagtg
     5341 aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac
     5401 ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc
     5461 cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt
     5521 tttaatcaaa catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc
     5581 tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa
     5641 tatcaacgct tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     5701 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5761 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccaaacgt actgcggtaa
     5821 agctcatcag cgtggtcgtg aagcaattc
//
