LOCUS pKLPBac 5849 bp DNA circular SYN 12-SEP-2012 DEFINITION . ACCESSION pKLPBac KEYWORDS . SOURCE Unknown. ORGANISM Unknown Unclassified. REFERENCE 1 (bases 1 to 5849) AUTHORS Self JOURNAL Unpublished. COMMENT SECID/File created by Clone Manager, Scientific & Educational Software COMMENT SECNOTES|GenBank 5849 bp ds-DNA circular 12-SEP-2012 ACCESSION VERSION FEATURES Location/Qualifiers misc_feature complement(1..816) /label=KanR /ApEinfo_fwdcolor=#ffff00 /ApEinfo_revcolor=#ffff00 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="KanR" rep_origin 862..1544 /label=ColE1 origin /ApEinfo_fwdcolor=gray50 /ApEinfo_revcolor=gray50 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="ColE1 origin" misc_feature 1831..2055 /label=Tn7R /ApEinfo_fwdcolor=#0064fc /ApEinfo_revcolor=#0064fc /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="Tn7R" misc_feature complement(2120..2872) /label=GentR /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Gene" /SECStyleId=1 /SECName="GentR" misc_recomb 3268..3301 /label=LoxP /ApEinfo_fwdcolor=steel blue /ApEinfo_revcolor=orange /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="LoxP" misc_feature 3333..3461 /label=Polh promoter /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=green /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="Polh promoter" misc_feature 3474..3476 /label=Start /ApEinfo_fwdcolor=#d6e9ff /ApEinfo_revcolor=#d6e9ff /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="Start" misc_feature 3480..4205 /label=TEV protease /ApEinfo_fwdcolor=cyan /ApEinfo_revcolor=cyan /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="TEV protease" misc_feature 4245..4265 /label=TEV site1 /ApEinfo_fwdcolor=#feff0c /ApEinfo_revcolor=#feff0c /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="TEV site1" misc_feature 4266..5009 /label=eCFP /ApEinfo_fwdcolor=#00ff13 /ApEinfo_revcolor=#00ff13 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="eCFP" polyA_signal 5102..5293 /label=SV40 late polyA /ApEinfo_fwdcolor=pink /ApEinfo_revcolor=pink /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="SV40 polyA" misc_feature 5328..5493 /label=Tn7L /ApEinfo_fwdcolor=#015ab8 /ApEinfo_revcolor=#015ab8 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 /SECDrawAs="Region" /SECStyleId=1 /SECName="Tn7L" ORIGIN 1 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat 61 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca 121 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc 181 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac 241 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca 301 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg 361 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga 421 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata 481 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc 541 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt 601 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa 661 caactctggc gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac 721 attatctcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg 781 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat 841 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact 901 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 961 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 1021 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 1081 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 1141 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 1201 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 1261 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 1321 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 1381 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 1441 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 1501 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 1561 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 1621 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 1681 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 1741 tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct ggcaaaatcg gttacggttg 1801 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 1861 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 1921 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 1981 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2041 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2101 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2161 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 2221 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 2281 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 2341 ctcactacgc ggctgctcaa acttgggcag aacgtaagcc gcgagagcgc caacaaccgc 2401 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 2461 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 2521 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 2581 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 2641 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 2701 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 2761 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 2821 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 2881 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 2941 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 3001 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg cccttgcttc 3061 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 3121 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 3181 atagttctag tggttggcta cagctttgtt tgtactatca acaggttgaa ctgctgatca 3241 acagatcctc tacgcggccg cggtaccata acttcgtata gcatacatta tacgaagtta 3301 tctggtttcg acctactccg gaatattaat agatcatgga gataattaaa atgataacca 3361 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat 3421 aaatattccg gattattcat accgtcccac catcgggcgc ggatcccatg ggcatgggcg 3481 gagaaagctt gtttaaggga ccacgtgatt acaacccgat atcgagcacc atttgtcatt 3541 tgacgaatga atctgatggg cacacaacat cgttgtatgg tattggattt ggtcccttca 3601 tcattacaaa caagcacttg tttagaagaa ataatggaac actgttggtc caatcactac 3661 atggtgtatt caaggtcaag aacaccacga ctttgcaaca acacctcatt gatgggaggg 3721 acatgataat tattcgcatg cctaaggatt tcccaccatt tcctcaaaag ctgaaattta 3781 gagagccaca aagggaagag cgcatatgtc ttgtgacaac caacttccaa actaagagca 3841 tgtctagcat ggtgtcagac actagttgca cattcccttc atctgatggc atattctgga 3901 agcattggat tcaaaccaag gatgggcagt gtggcagtcc attagtatca actagagatg 3961 ggttcattgt tggtatacac tcagcatcga atttcaccaa cacaaacaat tatttcacaa 4021 gcgtgccgaa aaacttcatg gaattgttga caaatcagga ggcgcagcag tgggttagtg 4081 gttggcgatt aaatgctgac tcagtattgt gggggggcca taaagttttc atgagcaaac 4141 ctgaagagcc ttttcagcca gttaaggaag cgactcaact catgaatgaa ttggtgtact 4201 cgcaaggtaa ccatagggat aacagggtaa tgcggtccgg tggtgaaaac ctgtattttc 4261 agggcgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 4321 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 4381 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 4441 ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga 4501 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 4561 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 4621 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 4681 acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga 4741 acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 4801 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 4861 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 4921 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt 4981 ccggactcag atctcgacga gctcactagt aatctagagc ctgcagtctc gacaagcttg 5041 tcgagaagta ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 5101 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 5161 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5221 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5281 cttatcatgt ctggatctga tcactgcttg agcgcctagg agatccgaac cagataagtg 5341 aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac gacgctacac 5401 ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc catttccacc 5461 cctcccagtt cccaactatt ttgtccgccc acagcggggc atttttcttc ctgttatgtt 5521 tttaatcaaa catcctgcca actccatgtg acaaaccgtc atcttcggct actttttctc 5581 tgtcacagaa tgaaaatttt tctgtcatct cttcgttatt aatgtttgta attgactgaa 5641 tatcaacgct tatttgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa 5701 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 5761 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccaaacgt actgcggtaa 5821 agctcatcag cgtggtcgtg aagcaattc //