13C assignments of Ca-loaded calbindin D9k at pH 6.0, 300 K. Jens Madsen (Chazin Lab) Frans Mulder (Akke Lab, Lund University, Sweden). 1996-06-06 Residue HA CO CA CB CG1 CG2 CD1 CD2 CE 0 M 4.27 176.658 - - - - 15.9 1 K 4.60 173.171 54.9 33.4 28.2 27.8 40.9 2 S 4.78 - 55.4 62.7 3 P 4.32 177.239 65.2 30.9 26.9 49.4 4 E 4.08 177.48 59.2 27.9 35.8 - 5 E 4.16 177.71 58.0 29.4 36.2 - 6 L 4.26 176.046 57.1 41.0 26.2 24.7* 22.9* 7 K 3.98 175.568 59.1 31.2 23.4 28.1 40.5 8 G 3.92,3.92 174.803 46.1 9 I 3.87 174.344 64.6 37.9 29.6 16.5 12.9 10 F 3.52 174.114 62.0 38.4 - - - 11 E 3.75 176.8 58.4 28.6 36.3 - 12 K 3.84 175.758 58.0 31.1 23.0 28.5 40.7 13 Y 4.00 174.019 60.6 38.0 - - - 14 A 3.80 176.658 53.3 16.1 15 A 4.30 175.25 51.8 18.0 16 K 3.85 174.841 59.2 31.6 24.1 27.7 40.7 17 E 4.72 173.789 53.2 32.2 33.8 - 18 G 3.62,3.95 171.685 45.2 19 D 4.69 - 51.7 - - 20 P 4.80 172.297 63.2 30.4 25.4 50.1 21 N 4.95 171.661 51.3 39.6 - 22 Q 5.12 171.685 54.3 35.0 34.5 - 23 L 5.48 174.229 52.4 43.6 24.8 27.7 23.4 24 S 4.83 172.928 55.4 65.0 25 K 3.46 175.798 60.6 29.9 23.9 27.9 40.5 26 E 3.96 177.334 58.2 28.3 36.8 - 27 E 3.97 176.555 58.4 29.5 36.9 - 28 L 4.07 175.319 57.0 39.8 26.6 22.4 26.5 29 K 3.62 175.3 59.7 30.8 23.2 28.2 40.4 30 L 4.03 176.564 56.8 40.9 25.7 23.1 $ 31 L 2.33 177.008 58.0 41.5 26.3 27.5 23.6 32 L 3.85 176.173 57.7 40.6 25.3 25.8* 22.5* 33 Q 3.81 175.109 58.1 27.9 33.5 - 34 T 4.05 173.177 64.6 69.0 20.6 35 E 4.21 174.516 55.5 29.3 33.4 - 36 F 5.21 - 54.7 41.2 - - - 37 P 4.16 177.232 64.6 30.0 26.4 49.1 38 S 4.22 173.942 59.6 61.8 39 L 4.16 175.969 56.4 41.0 - 23.1# 24.2# 40 L 4.38 174.784 53.8 40.1 25.7 22.0* 24.7* 41 K 4.38 174.439 55.0 31.9 24.4 27.8 40.8 42 G 4.10,4.30 172.453 44.6 43 G 4.13,3.87 172.5 44.6 44 S 4.74 172.135 57.6 63.2 45 T 4.15 172.775 60.6 70.3 20.7 46 L 3.92 175.706 57.0 40.1 26.2 21.9# 24.4# 47 D 4.19 176.295 57.0 39.4 - 48 E 4.02 177.1 58.0 28.6 35.8 - 49 L 4.19 176.0 56.9 41.1 26.5 23.0 24.3 50 F 3.64 173.942 62.6 38.7 - - - 51 E 3.97 175.959 57.9 28.3 35.1 - 52 E 3.89 175.935 57.6 28.4 34.7 - 53 L 4.15 176.975 54.8 42.6 25.4 25.6 22.1 54 D 4.56 174.764 51.9 37.0 - 55 K 4.05 175.74 57.4 31.9 23.8 27.5 40.8 56 N 4.82 173.904 50.9 35.9 - 57 G 3.84,3.84 172.527 46.8 58 D 4.64 175.3 52.3 39.6 - 59 G 3.71,4.27 170.576 45.2 60 E 5.13 173.541 52.7 33.2 34.6 - 61 V 5.10 173.273 60.5 32.2 21.3 22.7 62 S 4.88 172.661 56.4 65.3 63 F 3.28 175.317 60.8 37.0 - - - 64 E 3.87 178.15 59.0 28.5 35.5 - 65 E 4.07 177.312 57.8 28.7 36.7 - 66 F 3.96 173.56 59.2 39.1 - - - 67 Q 3.49 175.128 57.0 26.8 32.8 68 V 3.56 175.467 64.7 30.8 20.0 21.5 69 L 3.78 175.549 55.9 40.2 25.4 24.2* 23.3* 70 V 3.08 175.434 65.4 30.1 20.0 22.2 71 K 3.98 175.584 57.5 31.0 24.0 28.1 40.6 72 K 4.12 175.489 57.0 32.1 - 28.1 40.3 73 I 4.01 173.808 61.3 37.4 26.3 16.7 12.1 74 S 4.50 170.901 57.9 63.1 75 Q 4.15 - 57.3 30.1 33.1 - $: 13C methyl not assigned due to degeneracy of 1H chemical shifts, (also 13C degen.?) *: Stereospecific assignment not available (protons distinct, CD1 arbitrarily denotes the 13C bound to the upfield proton) #: Stereospecific assignment not available (protons degenerate) These assignments were made based on CBCA(CO)NH (alphas and betas), HNCA + HSQC experiments (methyls and Ser2-alpha), and HCCH-TOCSY (other carbons). 13C side chain carbonyl assignments and 15N assignments can be found in other files. Updated 2002-02-12