Amino Acid Composition of the Calcium-Binding Loop in Canonical EF Hands


276 canonical calcium binding loops were examined. 165 unique sequences were identified. The following table shows how often each residue was found in each site in the binding loop. This table was taken from:

Marsden, BJ, GS Shaw, and BD Sykes. (1990) Biochem. Cell. Biol. 68. 587-601. (Table2)


Loop Position
Amino Acid 1 2 3 4 5 6 7 8 9 10 11 12
X * Y * Z G # I x * * z
asp 162 1 120 5 93 3 4 --- 55 1 44 15
asn 1 2 40 15 28 3 4 1 10 2 7 2
glu 1 9 4 5 2 --- 10 --- 23 16 52 142
gln --- 14 --- 7 1 --- 9 --- 2 3 8 1
ser --- 9 1 --- 37 1 10 --- 32 6 5 ---
thr --- 16 --- --- 1 1 21 1 18 9 3 ---
gly --- 2 --- 84 3 147 2 1 17 6 1 ---
lys --- 46 --- 23 --- 3 22 --- --- 10 14 ---
arg --- 13 --- 10 --- 2 5 --- 2 6 ---
his --- --- --- 7 --- 2 1 1 --- --- --- ---
ala --- 16 --- 8 --- 1 6 1 1 17 17 ---
val --- 13 --- --- --- --- 6 21 1 19 1 4
leu --- 5 --- --- --- 2 5 15 1 16 1 1
ile --- 10 --- --- --- --- 4 116 --- 6 1 ---
tyr 1 1 --- --- --- --- 24 --- --- 13 2 ---
phe --- 4 --- --- --- --- 26 --- --- 27 --- ---
cys --- 2 --- --- --- --- 2 3 1 --- --- ---
met --- 2 --- 1 --- --- 3 4 2 1 2 ---
pro --- --- --- --- --- --- 1 1 --- 5 7 ---
trp --- --- --- --- --- --- --- --- --- 2 --- ---

Also noted in the same paper:



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