Information in the EF-Hand Calcium-Binding Proteins Database from Reference 629

Protein	Species	Information	Comment	Submitted By	Validated By
S100A12	Sus scrofa (pig)	This protein binds 2 equivalents of Ca ion	S100A12 binds two equivalents of calcium in the presence of zinc, but seems to only bind one equivalent of calcium with high affinity in the absence of zinc	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
S100A12	Sus scrofa (pig)	This protein binds 1 equivalents of Zn ion		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
S100A12	Sus scrofa (pig)	Ca binding constants measured by flow dialysis: K₁ = 19000 +/- 4000 Temperature: 20 pH: 7.4 Salt concentration: no salt added Buffer: 0.025 M Tris-HCl	The existence of an additional low affinity calcium binding site could not be proven nor disproven in this study	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
S100A12	Sus scrofa (pig)	Ca binding constants measured by flow dialysis: K₁ = 2.7E07 +/- 3E06 K₂ = 65000 +/- 12000 Temperature: 20 pH: 7.4 Salt concentration: no salt added Buffer: 0.025 M Tris-HCl 1e-04 M ZnCl2		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu