Information in the EF-Hand Calcium-Binding Proteins Database from Reference 614

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 614

Protein	Species	Information	Submitted By	Validated By
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M L6V	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M F10A	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M L23A	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M L23G	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M L28A	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M V61A	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M F66W	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M F66A	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M V70L	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M I73V	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k	Bos taurus (cow or bovine)	Report of an engineered mutant with following mutations: P43M V61G	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L6V	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 4.35 +/- 0.24 Cooperativity of transition: 1 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F10A	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 1.72 +/- 0.12 Cooperativity of transition: 0.88 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23A	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 2.82 +/- 0.14 Cooperativity of transition: 1.03 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L23G	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 2.27 +/- 0.31 Cooperativity of transition: 1.22 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M L28A	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 3.85 +/- 0.17 Cooperativity of transition: 1.03 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61A	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 2.77 +/- 0.17 Cooperativity of transition: 0.81 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66W	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 4.2 +/- 0.33 Cooperativity of transition: 0.69 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M F66A	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 1.58 +/- 0.12 Cooperativity of transition: 0.79 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V70L	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 5.35 +/- 0.24 Cooperativity of transition: 0.81 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M I73V	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 5.02 +/- 0.26 Cooperativity of transition: 1.15 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M V61G	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 2.2 +/- 0.19 Cooperativity of transition: 0.86 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calbindin D9k mutant: P43M this mutant is often used in NMR studies to substitute for wildtype, because it removes the problem of two sets of signals arising due to cis-trans isomerization at P43	Bos taurus (cow or bovine)	Stability measured by urea denaturation: 6.55 +/- 0.31 Cooperativity of transition: 1.17 kcal/(mol M) Temperature: 25 pH: 7 Buffer: 0.0005 M EGTA 0.01 M potassium phosphate	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu