Information in the EF-Hand Calcium-Binding Proteins Database from Reference 499

Protein	Species	Information	Comment	Submitted By	Validated By
parvalbumin beta lineage	Cyprinus carpio (carp)	Ca binding constants measured by equilibrium dialysis: K₁ = 2.7E09 K₂ = 2.7E09 Temperature: 25 pH: 7.4 Salt concentration: 80 mM KCl Buffer: 0.025 M Tris-HCl 1e-04 M dithiothreitol	Binding constants were measured by the equilibrium dialysis method.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
parvalbumin beta lineage	Cyprinus carpio (carp)	This protein binds 2 equivalents of Ca ion		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
parvalbumin beta lineage	Cyprinus carpio (carp)	This protein binds 2 equivalents of Mg ion		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
parvalbumin beta lineage	Cyprinus carpio (carp)	Mg binding constants measured by equilibrium dialysis: K₁ = 95000 K₂ = 95000 Temperature: 25 pH: 7.4 Salt concentration: 80 mM KCl Buffer: 0.025 M Tris-HCl 1e-04 M dithiothreitol		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu