| Protein |
Species |
Information |
Comment |
Submitted By |
Validated By |
|---|---|---|---|---|---|
| calbindin D9k |
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 4.78 +/- 0.24 Cooperativity of transition: 0.908 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 3.58 +/- 0.24 Cooperativity of transition: 0.884 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 5.26 +/- 0.24 Cooperativity of transition: 0.908 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 3.58 +/- 0.24 Cooperativity of transition: 0.788 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 3.58 +/- 0.24 Cooperativity of transition: 0.86 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Stability measured by urea denaturation: 4.19 +/- 0.24 Cooperativity of transition: 0.836 kcal/(mol M)
|
Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Calcium-loaded form is slightly less stable to pH compared to wildtype | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Apo state is slightly more stable to urea denaturation than wildtype apo state is, probably due to a decrease in electrostatic repulsion at the binding site | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Apo state is slightly less stable to urea denaturation than apo state of wildtype is. | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Apo state is slightly less stable to urea denaturation than apo state of wildtype is. Apo is state is also slightly less stable than apo state of P20G | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |
|
| calbindin D9k mutant:
|
Bos taurus (cow or bovine) |
Apo state is slightly less stable to urea denaturation than apo wildtype protein is. Urea denaturation curve is almost identical to the curve for P20G alone | Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu |
Melanie R. Nelson mnelson@scripps.edu |