Information in the EF-Hand Calcium-Binding Proteins Database from Reference 455

Protein	Species	Information	Comment	Submitted By	Validated By
caltractin isoform 1	Chlamydomonas reinhardtii (green algae)	This protein binds 4 equivalents of Ca ion		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
caltractin isoform 1	Chlamydomonas reinhardtii (green algae)	Ca binding constants measured by flow dialysis: K₁ = 830000 K₂ = 830000 K₃ = 6250 K₄ = 6250 Temperature: 25 pH: 7.5 Salt concentration: 75 mM KCl Buffer: 0.05 M HEPES	The binding constants were measured by the calcium electrode method. The protein concentration was 50 micromolar.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
caltractin isoform 1	Chlamydomonas reinhardtii (green algae)	Ca binding constants measured by flow dialysis: K₁ = 280000 K₂ = 280000 K₃ = 1750 K₄ = 1750 Temperature: 25 pH: 7.5 Salt concentration: 75 mM KCl Buffer: 0.05 M HEPES 0.005 M MgCl2	The binding constants were measured by the calcium electrode method. The protein concentration was 50 micromolar.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu

Protein

Species

Information

Comment

Submitted By

Validated By

caltractin
isoform 1

Chlamydomonas reinhardtii
(green algae)

This protein binds 4 equivalents of Ca ion

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu

caltractin
isoform 1

Chlamydomonas reinhardtii
(green algae)

Ca binding constants measured by flow dialysis:

The binding constants were measured by the calcium electrode method. The protein concentration was 50 micromolar.

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu

caltractin
isoform 1

Chlamydomonas reinhardtii
(green algae)

Ca binding constants measured by flow dialysis:

The binding constants were measured by the calcium electrode method. The protein concentration was 50 micromolar.

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu