CaBP Data Library General Information

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 364

Protein
 Species
 Information
 Comment
 Submitted By
 Validated By
calmodulin-like protein
 Homo sapiens
(human)
 This protein binds 4 equivalents of Ca ion
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
 Melanie R. Nelson
mnelson@scripps.edu
calmodulin-like protein
 Homo sapiens
(human)
 Calculated pI: 4.1
    		 This pI was calculated by the PEPTIDESORT program in the UWGCC package (Devereux, J. P. Haeberli, O. Smithies (1984) Nucleic Acids Res 12. 387-395.).
    		 Melanie R. Nelson
    The Scripps Research Institute, Department of Molecular Biology
    mnelson@scripps.edu
    		 Melanie R. Nelson
    mnelson@scripps.edu
    calmodulin
    isoform 1
    the main isoform, and the only isoform found in most species
    		 Homo sapiens
    (human)
    		 Calculated pI: 3.9
      		 This pI was calculated by the PEPTIDESORT program in the UWGCC package (Devereux, J. P. Haeberli, O. Smithies (1984) Nucleic Acids Res 12. 387-395.)
      		 Melanie R. Nelson
      The Scripps Research Institute, Department of Molecular Biology
      mnelson@scripps.edu
      		 Melanie R. Nelson
      mnelson@scripps.edu
      calmodulin-like protein
      		 Homo sapiens
      (human)
      		 Ca binding constants measured by flow dialysis:
      • K1 = 380000 +/- 40000
      • K2 = 190000 +/- 20000
      • K3 = 49000 +/- 10000
      • K4 = 12000 +/- 3000
      • Temperature: 25
      • pH: 7.5
      • Salt concentration: no salt added
      • Buffer:
        • 0.03 M HEPES
        • 0.002 M MgCl2
        • 0.0075 M beta-mercaptoethanol
        • 0.03 M imidazole
      		 These stoichiometric binding constants were measured using the flow dialysis method at 25 degrees Celsius. The protein was 70 micromolar.
      		 Melanie R. Nelson
      The Scripps Research Institute, Department of Molecular Biology
      mnelson@scripps.edu
      		 Melanie R. Nelson
      mnelson@scripps.edu
      calmodulin
      isoform 1
      the main isoform, and the only isoform found in most species
      		 Homo sapiens
      (human)
      		 Only 23 amino acids differ between calmodulin and calmodulin-like protein
      		Melanie R. Nelson
      The Scripps Research Institute, Department of Molecular Biology
      mnelson@scripps.edu
      		 Melanie R. Nelson
      mnelson@scripps.edu