CaBP Data Library General Information

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 263

Protein
Species
Information
Comment
Submitted By
Validated By
calbindin D9k
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 1.6E08
  • K2 = 4E08
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: no salt added
  • Buffer:
    • 0.002 M Tris-HCl
The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. An earlier report from the same lab gives slightly different calcium binding constants. This is because the earlier report used partially deamidated protein. The reference for the earlier report is: Linse, S., T. Drakenberg, E. Thulin, P. Sellers, K. Elmden, T. Grundstrom, S. Forsen. Structure-function relationships in EF-Hand Ca2+-binding proteins. Protein engineering and biophysical studies of calbindin D9k (1987) Biochemistry. 26, 6723-6735.
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 5E06
  • K2 = 1.6E07
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 50 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl
The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. Protein concentrations were between 20 and 30 micromolar (the exact concentration is determined as part of the fitting of the titration curve.)
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 3.2E06
  • K2 = 6.3E06
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 100 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl
The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. Protein concentrations were between 20 and 30 micromolar (the exact concentration is determined as part of the fitting of the titration curve.)
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 2E06
  • K2 = 3.2E06
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 150 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl
The calcium affinity was determined using the competitive chelator method. The chelator used was Quin2. Protein concentrations were between 20 and 30 micromolar (the exact concentration is determined as part of the fitting of the titration curve.)
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • E17Q
Bos taurus
(cow or bovine)
This protein binds 2 equivalents of Ca ion
Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • E17Q
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 2.5E07
  • K2 = 1.26E08
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: no salt added
  • Buffer:
    • 0.002 M Tris-HCl

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • E17Q
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 3.16E06
  • K2 = 1E07
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 50 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • E17Q
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 2E06
  • K2 = 3.16E06
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 100 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu
calbindin D9k
mutant:
  • E17Q
Bos taurus
(cow or bovine)
Ca binding constants measured by competitive chelator:
  • K1 = 1.58E06
  • K2 = 2.51E06
  • Temperature: 25
  • pH: 7.5
  • Salt concentration: 150 mM KCl
  • Buffer:
    • 0.002 M Tris-HCl

Melanie R. Nelson
The Scripps Research Institute, Department of Molecular Biology
mnelson@scripps.edu
Melanie R. Nelson
mnelson@scripps.edu