Information in the EF-Hand Calcium-Binding Proteins Database from Reference 262

Information in the EF-Hand Calcium-Binding Proteins Database from Reference 262

Protein	Species	Information	Comment	Submitted By	Validated By
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1E07 K₂ = 3.98E07 K₃ = 3.16E06 K₄ = 2.5E06 Temperature: 25 pH: 7.5 Salt concentration: no salt added Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 1.58E06 K₂ = 2.5E07 K₃ = 1E06 K₄ = 1E06 Temperature: 25 pH: 7.5 Salt concentration: 10 mM KCl Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 630000 K₂ = 1E07 K₃ = 250000 K₄ = 500000 Temperature: 25 pH: 7.5 Salt concentration: 25 mM KCl Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 250000 K₂ = 5E06 K₃ = 39800 K₄ = 316000 Temperature: 25 pH: 7.5 Salt concentration: 50 mM KCl Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 79000 K₂ = 3.98E06 K₃ = 25000 K₄ = 398000 Temperature: 25 pH: 7.5 Salt concentration: 100 mM KCl Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	Ca binding constants measured by competitive chelator: K₁ = 50000 K₂ = 1.58E06 K₃ = 10000 K₄ = 200000 Temperature: 25 pH: 7.5 Salt concentration: 150 mM KCl Buffer: 0.002 M Tris-HCl	Binding constants were measured using the competitive chelator method, with 5,5'-Br₂BAPTA as the competitive chelator.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
calmodulin isoform 1 the main isoform, and the only isoform found in most species	Bos taurus (cow or bovine)	This protein binds 4 equivalents of Ca ion		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu