Information in the EF-Hand Calcium-Binding Proteins Database from Reference 16

Protein	Species	Information	Comment	Submitted By	Validated By
recoverin	Bos taurus (cow or bovine)	structure by NMR (pdb entry 1JSA) of full length protein bound to 2 ions of Ca		Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu
recoverin	no species information	The conformational change between apo and two calcium ions bound states involves: Calcium-binding leads to the exposure of the covalently attached myristoyl group to solvent. This is accomplished by rotation of a "hinge" at Gly42, which moves helix I of the first EF_hand, pulling the N-terminal helix away from the rest of the protein, and exposing the myristoyl group.	This paper reports the NMR strucutre of fatty-acylated recoverin in the calcium-loaded state. The N-terminal fatty acyl group is a homolog of myristate, used to improve the solubuility of the protein in the calcium-bound form.	Melanie R. Nelson The Scripps Research Institute, Department of Molecular Biology mnelson@scripps.edu	Melanie R. Nelson mnelson@scripps.edu