CaBP Data Library General Information

SPARC/BM-40

Primary information about SPARC/BM-40:
Basic chemical and physical information
Basic biological information
Basic genomic information
Metal ion binding constants
Interspecies sequence alignment
Information on function
Summary of structural studies
Information on the conformational change List of available structures Information about mutations
References about SPARC Other web resources about SPARC

Basic Information

Additional names and abbreviations: SPARC: secreted protein rich in cysteines (SPARC); osteonectin; 43K protein
Isoforms: No information about isoforms is stored in the database
Yeast Protein Database Entries: No links to the YPD are stored in the database


Basic Chemical and Physical Information:
Number of amino acids: 10 (Oryctolagus cuniculus) - 304 (Bos taurus)
View a list of all sources stored in the database
Molecular weight: 1.097 kD (Oryctolagus cuniculus) - 34.716 kD (Bos taurus)
View a list of all sources stored in the database
pI: calculated pI (Homo sapiens) = 4.7 (InfoCard)
Number of functional calcium binding sites: Homo sapiens: 2 (InfoCard)
Macroscopic calcium binding constants: not yet available in the data library
Other metal binding constants: not yet available in the data library
Protein stability: not yet available in the data library
Post-translational modifications: Bos taurus: glycosylation (InfoCard)
Homo sapiens: glycosylation (InfoCard)


Basic Biological Information:
Source organisms: Bos taurus, Caenorhabditis elegans, Gallus gallus, Homo sapiens, Mus musculus, Sus scrofa, Oryctolagus cuniculus, Rattus norvegicus, Xenopus laevis
View an annotated list of all sources stored in the database
Tissues: Homo sapiens: embryonic developing bone (InfoCard)
Homo sapiens: embryonic developing teeth (InfoCard)
Subcellular localization: Homo sapiens: extracellular (InfoCard)
Regulation: not yet available in the data library


Genomic Information:
GenBank entries: View a list of GenBank entries stored in the database
Gene length: not yet available in the data library
Gene structure: Mus musculus, expressed: GenBank code X12697; 9 introns (InfoCard)
Caenorhabditis elegans, expressed: GenBank code L21758; 5 introns (InfoCard)
Promoter: not yet available in the data library
Gene copies: not yet available in the data library
Alleles: no alleles are currently stored in the data library
Chromosomal localization: Homo sapiens, expressed (GenBank code J03040): q31-q33 region of chromosome 5 (InfoCard)
Mus musculus, expressed (GenBank code X12697): sub-band B1 region of chromosome 11 (InfoCard)
Caenorhabditis elegans, expressed (GenBank code L21758): chromosome 4 (InfoCard)

Interspecies Sequence Alignment



BOVINE  : MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVAEVPVGANPVQVEVGEFDDGAE
HUMAN   : MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAE
MOUSE   : MRAWIFFLLCLAGRALAAPQQ-TEVAEEIVEEETVVEETGVPVGANPVQVEMGEFEDGAE
RAT     : MRAWIFFLLCLAGRALAAPQ--TEAAEEMVAEETVVEETGLPVGANPVQVEMGEFEEGAE
CHICK   : MRTWIFFFLCLAGKALAAPQE-ALADETEVIEDLTTEG---PVGANPVQVEVGEFEE-PT
XENOPUS : MRVWVFFVLCLAGKALAAPQQDALPEEEEVIEDVPAEET---VGTNPVQVDVGEFDEAIN
CELEG   : MR--YALAACLL--LLAASSF-VDAKKKKIADDELGEL-------------LDNIDA--D

BOVINE  : ETEEEVVA-ENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGE-FEKVCSNDNKT
HUMAN   : ETEEEVVA-ENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGE-FEKVCSNDNKT
MOUSE   : ETVEEVVA-DNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGE-FEKVCSNDNKT
RAT     : ETPEEVVACNNPCQNHHCKHGKVCD-GESNTPMCVCQDPTSCPAPIGE-FEKVCSNDNKT
CHICK   : EDVEEIVA-ENPCQNHHCKHGKVCEVDDNNSPMCVCQDPSSCPAHSGV-FEKVCGTDNKT
XENOPUS : EEEEEEPS-ENPCLNHHCKHGKVCEVDESNTPLCVCQDPSTCPTSVGE-FEKICGTDNKT
CELEG   : EEKKSVEPAKNPCEDHQCGWGKECVVGKKGEPTCECI--SKCPELDGDPMDKVCANNNQT

BOVINE  : FDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLK
HUMAN   : FDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLK
MOUSE   : FDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLK
RAT     : FDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLK
CHICK   : YDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKFIPACLDTELTEFPLRMRDWLK
XENOPUS : YDSSCHFFATKCTLEGTKK------GHKLHLDYIGPCKYIAPCLDNELSEFPLRIGDWLK
CELEG   : FTSLCDLYRERCLCKRKSKECSKAFNAKVHLEYLGECKKLDECTEEHMAQFPERMADWLF

BOVINE  : NVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGCDHPVELLARDFEKNYNMYIF
HUMAN   : NVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAG-DHPVELLARDFEKNYNMYIF
MOUSE   : NVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAG-DHPVELLARDFEKNYNMYIF
RAT     : NVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAG-DHPVELLARDFEKNYNMYIF
CHICK   : NVLITLYERDEDNNLLTEKQKLKVKNIHENEKRLEAG-DHTVELLARDFEKNYNMYIF
XENOPUS : NVLVSLYERDENNNLLNEKQKLRVKKIHENEKRLESW-RPHSELLVRDFEKNYNMYIF
CELEG   : QVMKELKKRRE---------------LH----KLEWE-----ELLSEAENDDEKKHVY

          |-helixI-|--loop1----|helixII-|    |helixIII|--loop2----|helixIV|
          En**nn**nX*Y*ZG#Ix**zn**nn**n*     En**nn**nX*Y*ZG#Ix**zn**nn**n* 

BOVINE  : PVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKE
HUMAN   : PVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQ
MOUSE   : PVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKE
RAT     : PVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKE
CHICK   : PVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFEACDLDFDKYIALEEWASCFGIKE
XENOPUS : PVHWQFGQLDQHPIDGYLSHTELSPLRAPLIPMEHCTTRFFDECDIDDDKYIALEEWAKCFGIKE
CELEG   : PVIWKFCELDTKPHDKSVSHHELIPITAPVIPMESCIKPFLEGCDANNDGNISIKEWGKCLGIKE
BOVINE  : KDIDKDLVI
HUMAN   : KDIDKDLVI
MOUSE   : QDINKDLVI
RAT     : QDINKDLVI
CHICK   : QDIDKDLVI
XENOPUS : QDVDKDMIV
CELEG   : GEIQERC--


Sequence alignment for entire SPARC subfamily

Functional Information:

General information:

Biological roles:

Disease states:

Target molecules:


Structural Information:

Calcium-coordination:
not yet available in the data library


Nature of the conformational changes:
not yet available in the data library


Secondary structure:
(as reported in the PDB file and its accompanying reference)
not yet available in the data library


Additional structural information:


Available structures:
(follow the links from the PDB code to the retrieve the
PDB files)
Calcium Loaded Structures:
1SRA: Homo sapiens, extracellular calcium-binding domain, pH 7.5
Crystal structure, 2 angstrom resolution, R-value = 19.6, R-free = 24.9
2 Ca ions bound
(InfoCard)
1BMO: Homo sapiens, extracellular calcium-binding domain and follistatin-like domain, pH 7.5
Crystal structure, 3.1 angstrom resolution, R-value = 26.2, R-free = 32.2
2 Ca ions bound
(InfoCard)

Information about mutants: