Calmodulin Mutant: F92A

Engineered mutant of isoform 1 calmodulin, from Homo sapiens (InfoCard)

General Effects of the Mutation:

• This mutation is sufficient to cause a temperature-sensitive phenotype in yeast (InfoCard)
• This mutation causes a decrease in the thermal stability of the isolated Cam-C domain, as indicated by a 11.8 degree Celsius shift in the far-UV CD thermal dentauration curve. It also increases the calcium affinity. The calcium-dependent increase in bisANS fluorescence is 45% less in CaM-Ca with the F92A mutation than in wildtype CaM-C. This fluorescence increase monitors how hydrophobic the environment of the probe is-- a larger increase corresponds to a more hydrophobic environment (InfoCard)

Physical Properties of the Mutant:

No physical properties of this mutant are currently stored in the database