Information from the EF-Hand CaBP Database about Calbindin D9k Mutant Y13F

Calbindin D9k Mutant: Y13F

Engineered mutant of calbindin D9k, from Bos taurus (InfoCard)

General Effects of the Mutation:

Apo form is slightly less stable to urea and temperature unfolding compared to wildtype (InfoCard)
Calcium-loaded form is slightly less stable to pH compared to wildtype (InfoCard)

Physical Properties of the Mutant:

Stability:
Free energy of unfolding = 3.58 +/- 0.24 kcal/mol
cooperativity of unfolding = 0.884 kcal/(mol M)
(measured by: urea denaturation pH: 7.1 temperature: 25 degrees Celsius buffer: 0.001 M EGTA, 0.02 M Pipes )
(InfoCard)

Ca Binding Constants (low salt:) :

K₁ = 3.7 x 10⁸ +/- 5 x 10⁷; K₂ = 2.5 x 10⁸ +/- 4 x 10⁷
(measured by: competitive chelator pH: 7.5 temperature: 25 degrees Celsius buffer: 0.002 M Tris-HCl )
(InfoCard)